A simple, sensitive, rapid, accurate, precise and economical Q-absorbance ratio method has been developed for the simultaneous determination of Amitryptiline HCl and Chlordizepoxide in combined dosage form. Absorbance ratio method uses the ratio of absorbances at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components. Amitryptiline HCl and Chlordizepoxide show an isoabsorptive point at 220 nm in 0.1 N HCl. The second wavelength used is 246 nm, which is the λ-max of Chlordizepoxide in 0.1 N HCl. The linearity was obtained in the concentration range of 5-10 μg/ml for Amitryptiline HCl and 5-10 μg/ml for Chlordizepoxide. The concentrations of the drugs were determined by using ratio of absorbances at isoabsorptive point and at the λ-max of Chlordizepoxide. The method was successfully applied to pharmaceutical dosage form because of no interference. The results of analysis have been validated by recovery studies.
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